A, structure of BODIPY-cholesterol with the fluorophore (green, with light green underlay to highlight the fluorescent group) at carbon 24 of the sterol side chain. from Invitrogen (Carlsbad, CA). In this study, the validated BODIPY-cholesterol assay was used for the first time in ram spermatozoa to quantify cholesterol efflux by tracking the loss of BODIPY-cholesterol from the sperm plasma membrane . Aberrant Shh pathway activation is involved in the development of many To achieve maximum embedding of BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorophore into the bilayer apolar region, a series of sn-2 acyl-labeled phosphatidylcholines was synthesized bearing 4,4-difluoro-1,3,5,7-tetramethyl-4 . 1 A) and demonstrated that it partitions into the L o phase of bilayers. • A two-step model fits the kinetics of transport of BODIPY-cholesterol to endosomes. Structures of Bodipy-cholesterol, Bodipy-cholesteryl oleate, and ... 1, 2 it co-localizes with dehydroergosterol, a marker of cholesterol, in hela cells and is trafficked from the plasma membrane to the endocytic recycling compartment in bhk cells. Invitrogen™ Low Density Lipoprotein from Human Plasma, BODIPY™ FL complex (BODIPY™ FL LDL) Nonacetylated conjugate Manufacturer: Invitrogen™ L3483 Catalog No. Thus, the advent of dehydroergosterol [ergosta-5,7,9(),22-tetraen-3β-ol, DHE] the fluorescent sterol most structurally and functionally similar to cholesterol to date, has proven to be a major asset for real-time probing/elucidating the sterol environment and intracellular sterol . BODIPY‐Cholesterol: A New Tool to Visualize Sterol ... - DeepDyve . a cholesterol-Bodipy (Bdp-Chol) derivative (Fig. Cellular free cholesterol concentrations were measured using an Amplex™ Red cholesterol assay kit (Invitrogen) according to manufacturer's instructions. (HEK293) cells and pulsing the cells the following day with BODIPY-labeled LDL (Invitrogen). Bodipy-LDL fluorescence is neither quenched at high concentration nor reduced by acidic pH as in the lysosomes to which LDL-bound LDLRs traffic, so it can be used to quantify intracellular LDL . Taking people of the lipophilic BODIPY 493503 a lipid droplet-specific fluorescent dye described here where a detailed protocol for the. Differences to cholesterol are indicated in green and red; i.e., an extra methyl group and double bond in the alkyl side chain and two additional double bonds . Buffer medium contained 150 mM NaCl, 5 mM KCl, 1 mM . New BODIPY lipid probes for fluorescence studies of membranes Serum cholesterol levels were analyzed on an MIRA analyzer (Roche). B) M19 CHO cells were cultivated in the absence or presence of sterols supplied from DMSO as indicated and the protein amounts determined.
